International Fungal Multi Locus Sequence Typing Database
MLST, using partial sequence analysis of seven to ten housekeeping genes, has become the number one typing approach for epidemiological investigations of microorganisms. For each locus studied, different genetic sequences present within a species are assigned as distinct alleles. The combination of the identified alleles at each of the loci defines the allelic profile or sequence type for each isolate. The data generated can be used to determine whether the fungal isolates are clonal or have undergone recombination. As MLST is a sequence-based technique resulting in unambiguous, reproducible results it can be used to compare strains via a publicly available electronic network. However, the ability of the MLST analysis to give high strain discrimination relies on the choice of genes or loci selected in each study. Accurate sequence determination and comparison is crucial as a single base difference denotes a different haplotype.
The purpose of this webpage is to make MLST schemes publicly available for the following human pathogenic fungal species:
Cryptococcus neoformans/Cryptococcus gattii
Scedosporium aurantiacum, Scedosporium apiospermum, Pseudallescheria boydii
Wieland Meyer Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Sydney Medical School-Westmead Hospital, Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Westmead Millennium Institute, Sydney, Australia