Table 1. MLST gene locus and primers information  
Locus Locus Desription Primer  Sequence (5' to 3') # base-pairs analyzed Analyzed sequence fragment start and end points
BRN1 Melanin biosynthesis reductase F: TAT TGG AAA GGC TAT GGC CA                        R: GGC AGA CAG CGT GGT ACA T 485 5' TACGCCAA… …AGAAG(G/A)TC 3'
EF1a Elongation factor-1 alpha F: TCG GTG TCA AGC AGC TCA T                           R: AAT CTT CTC GAG GAG CTC GG 569 5'GAGGAGCG… …CAGGTCGG 3'
GPDH Glyceraldehyde-3-phosphate dehydrogenase F: GTC TCG CAT GCG TAG GTG T                                R: AGT GGT TGT GCA GGA GGC 448-453 5' TCCATTGA… …CGTCATGG 3'
RPB1 Large subunit of RNA polymerase-like F: TAC AAC CTG GCT ACC CCG                             R: CAA GAG CCT GCT TCT TCT TGT T 556 5'TTCTCC(T/A)G… …C(G/A)TT(C/T)TTC 3'
RPB2 Small subunit of RNA polymerase-like F: GTG GAA AAC AAC CAA GAC TTC AA              R: ATA TCA CGA ATC AAA CTC ATC TCG T 437 5' CGGC(C/T)TGA… …(A/G)GT(C/T)GTGC 3'
SAL1 Scytalone dehydratase  F: CAT GGC TAA CTA GTT TGC AGA TGT              R: CCG CGA CTC ATC CGT GTA T 275 5' G(G/T)CGGC(A/G/C)T… …TCA(C/T)CA (G/A)C 3'
PCR settings for all loci:  94°C for 5 min; 30 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min; and a final elongation step at 72°C for 2 min.