International Fungal Multi Locus Sequence Typing Database
The MLST scheme for genotyping Pneumocystis jirovecii is based on sequence analysis of four genetic loci: the internal transcribed spacer 1 and 2 (ITS1/2) regions including the 5.8S rRNA gene of the nuclear rRNA gene cluster, and the P. jirovecii-specific ß-tubulin (ß-Tub), dihydropteroate synthase (DHPS) and mitochondrial large subunit rRNA (mtLSU) genes.
The loci are amplified using primers and amplification conditions as specified in:
Phipps LM, Chen SCA, Kable K, Halliday CL, Firacative C, Meyer W, Wong G, Nankivell BJ. Nosocomial Pneumocystis jirovecii pneumonia: lessons from a cluster in kidney transplant recipients. Transplantation. 2011;92(12):1327-1334.
Gianella S, et al. Molecular evidence of interhuman transmission in an outbreak of Pneumocystis jirovecii pneumonia among renal transplant recipients. Transplant Infectious Disease 2010;12:1-10.
Montes-Cano MA, et al. Pneumocystis jirovecii genotypes in the Spanish Population. CID 2004;34:123-8.
Prof. Wieland Meyer, BSc. Carolina Firacative (Molecular Mycology Research Laboratory, Sydney Medical School - Westmead Hospital, University of Sydney, Sydney, Australia)
Dr. Lisa M. Phipps, A/Prof. Sharon CA Chen, Kathy Kable, Dr. Catriona L. Halliday, Dr. Germaine Wong, Dr. Brian J. Nankivell (Department of Renal Medicine and Centre for Infectious Diseases and Microbiology, Westmead Hospital, Australia)
Wieland Meyer Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Sydney Medical School-Westmead Hospital, Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Westmead Millennium Institute, Sydney, Australia